Please use this form to recommend updates to the information in zfin. The sox10 antibody spm607 allophycocyanin has been validated for the following applications. To address these issues we employed a zebrafish line that was transgenic for an inducible inhibitor of wnt. The proneural transcription factor neurogenin 1 neurog1 has been shown to be a key regulator of dorsal root ganglion drg neuron development. Sox10 antibody clone 1e6 was used for ihcp staining of embryoid bodies which were generated differentiated from mouse p19 mouse embryonal carcinoma cell line using the hanging drop method wang and yang 2008, j visualized exp. Genetex currently offers a continuously expanding catalog of reagents designed expressly for zebrafish research, with more than 400 newly developed zebrafish antibodies. Mitf expression can be transactivated by both sox10 and pax3 to have an additive effect. Here we profiled the cell lineages labeled in the closely related neural crest transgenic lines tgfoxd3. These fish were crossed to generate embryos, in which foxd3 and sox10 transgenic neural crest labeling could be directly compared at the cellular level using live confocal imaging. Sox10 is also highly expressed in melanoma tumors, and sox10 expression increases with tumor progression.
Here we use a novel transgenic zebrafish line to demonstrate that the neural crest population that gives rise to drg neurons becomes fate restricted to a neuronalglial precursor before the onset of neurog1. Sox10 and pax3 interactions are thought to be regulators of other genes involved in the symptoms of waardenburg syndrome, particularly mitf which influences the development of melanocytes as well as neural crest formation. Due to their striking genomic similarity to humans, regenerative abilities, and shortened life cycle, zebrafish have become an ideal tool for studying developmental processes, gene expression, and underlying mechanisms of cancer and other diseases. Sox10 antibody sox21 antibody ws2e antibody ws4 antibody ws4c antibody zgc. The sox10 protein is widely expressed in normal human tissues including melanocytes and breast tissue. Sox10 protein staining sox10 antibodies biocare medical. Here, we establish a wholeanimalbased emt reporter in zebrafish for rapid drug screening, called tgsnai1b.
Genomic code for sox10 activation reveals a key regulatory. During development of wildtype ab zebrafish larva from 24 to 48 h postfertilization hpf, cells stained with anti sox10 antibody were associated closely with nerves, i. We appreciate as much detail as possible and references as appropriate. Sox10 srybox transcription factor 10 is a protein coding gene.
The transcription factor sox10 plays an important role in vertebrate neural crest development, including the establishment and maintenance of the melanocyte lineage. The sox10 antibody spm607 allophycocyanin from novus biologicals is a mouse monoclonal antibody to sox10. Rescue of neural crestderived phenotypes in a zebrafish charge model by sox10 downregulation. At 33 hpf, meis3 is expressed in the hindbrain, as well as in the posterior branchial arches and the developing fin buds arrowhead and arrow, respectively, in figure 1a. Sox10 expression is initiated in vagal ncc as they migrate from the neural tube toward the foregut and is responsible for the activation of ednrb and ret in encc once they enter the gut figure 12. Sox11 antibody sry sex determining region ybox 11 is a member of the sox family of transcription factors and is involved in the regulation of embryonic development and in the determination of cell fate 1. However, little is known about the sequences mediating transcriptional.
Primary spinal opc culture system from adult zebrafish to study oligodendrocyte differentiation in vitro. Hence, identifying compounds that block emt is essential to prevent or eradicate disseminated tumor cells. Chromatin immunoprecipitation chip assay of endogenous sox10 protein using antisox10 antibody. Analysis of gastrointestinal physiology using a novel. During zebrafish development, sox10 is expressed in the posterior region of the ppd, the anterior neural crest progenitor domain, and the entire otic placode and vesicle dutton et al. Flow cytometric analysis of hela cells using an isotype control red and sox9 d8g8h rabbit mab blue and a204 cells using sox9 d8g8h rabbit mab green. Diseases associated with sox10 include peripheral demyelinating neuropathy, central dysmyelination, waardenburg syndrome, and hirschsprung disease and waardenburg syndrome, type 2e. Requesting a zfin account or laboratory record a zfin account may be requested by contacting jonathan knight.
Sep 09, 2015 meis3 is expressed in the developing foregut environment during vagal and enteric neural crest cell migratory phases. Phenotypic chemical screening using a zebrafish neural. Sox10 zebrafish mutants to date, seven mutant alleles of zebrafish sox10 colourless, cls exist listed below. They are all recessive, and apparent hypomorphs or nulls. As the leading manufacturer of zebrafish antibodies, genetex offers an extensive portfolio of reagents validated for use in this model system. Sox10 lacz and sox10 dom mutant mice the sox10 locus was isolated from a 129sv genomic. Product description type unit quantity academic price book. The linearized targeting vector was electroporated into 129sv r1 es cells, which were selected with g418 and gancyclovir.
Transplantation of zebrafish pediatric brain tumors into. In vertebrates, the role of the enteric nervous system in regulating gastrointestinal contractions has been known for some time. Anatomy of zebrafish cerebellum and screen for mutations. The transplantation of tumor cells into immunecompromised animals, particularly mouse xenografts, is a widelyused technique used to study mechanisms controlling cancer cell proliferation 1, 2, survival, invasion, and metastasis 3, 4, as well as to provide a platform for screening drugs 5, 6, 7.
Dec 14, 2010 like other books in the laboratory animal pocket reference series, this guide covers all aspects pertaining to the use of these organisms including their basic biology, humane care and management, husbandry, life support systems, regulatory compliance, technical procedures, veterinary care, and water quality management. Anatomy of zebrafish cerebellum and screen for mutations affecting its development author links open overlay panel youngki bae a b 1 shuichi kani a 1 takashi shimizu a 1 koji tanabe a hideaki nojima a yukiko kimura c shinichi higashijima c masahiko hibi a d. We also conclude that sox10 is an important mediator of function of chd7 in nc. Both approaches converged on the 3 end of the conserved 1st intron as being critical for. In humans, mutations in the sox10 gene are a cause of the auditorypigmentary disorder waardenburg syndrome type iv ws4 and related variants. Abcam antibodies and reagents supplier, find any antibody. Sox10 antibody has been shown to be expressed in 97100% of desmoplastic and spindle cell melanomas and was also shown to be expressed in 100% of nevi. As with all cell fate decisions, transcriptional regulatory control plays a significant role in the progressive restriction and specification of nc derived lineages during development. Gfp, which labels epithelial cells undergoing emt to produce sox10 positive neural crest nc cells. Sox10 encodes an sryrelated hmg box protein essential for the development of the neural crest. Zebrafish antibodies primary antibodies sigmaaldrich. Interestingly, sox10 transcripts were also detected in cms in a recently published singlecell transcriptome of zebrafish embryos wagner et al. Materials and methods zebrafish lines and maintenance. Sox10 is indispensible for proper neural crest development and loss of sox10 leads to reductions of specific nc derivatives including peripheral neurons and melanocytes 21,22.
We have combined in vivo testing of dna fragments in zebrafish and computational comparative genomics to identify the first regulatory regions of the zebrafish sox10 gene. Ydbhs consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. The protein made by the sox10 gene directs the activity of other genes such as mitf that signal neural crest cells to become more specific cell types. Antisox10 melanoma marker monoclonal antibody bosterbio. Zebrafish dorsal root ganglia neural precursor cells adopt. Furthermore, it has been shown that tfap2a protein directly activates the expression of sox10 in zebrafish nccs van otterloo et al. Zebrafish is a powerful model system for investigating vertebrate neural development, in large part due to the rapid external development and transparency of the embryos. Antibodies, primary antibodies, z fish zebrafish, alphabetical listing of zebrafish antibodies. Sox10 directly modulates erbb3 transcription via an. If you cant find the antibody youre looking for, why not consider a custom reagent from one of these suppliers. Defects in sox10 are a cause of yemenite deafblind hypopigmentation syndrome ydbhs mim.
Egfp animals were labelled immunohistochemically with anti. Rescue of neural crestderived phenotypes in a zebrafish. Additional studies have shown that knockdown of both foxd3 and tfap2a in zebrafish completely ablates nccs and that these genes together are responsible for turning on key signaling pathways for neural. Thus, here we have shown a similar absence of glial differentiation, combined with expression of sensory neuron markers in residual drg neurons in zebrafish sox10 mutants. Pdf sox10 directly modulates erbb3 transcription via an.
Hello select your address best sellers gift ideas new releases whole foods todays deals amazonbasics coupons gift cards customer service free shipping shopper toolkit registry sell. Our boster quality guarantee for anti sox10 melanoma marker monoclonal antibody covers its use in the following applications. Elisa, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry, immunohistochemistryparaffin, western blot. Sox10 antibody has been shown to be expressed in 97100% of desmoplastic and spindle cell melanomas and was. To study the molecular basis of neural crest differentiation, we have cloned the gene disrupted in the mont blanc mobm610 mutation, which affects all neural crest derivatives.
The hybridomas are stored at the monoclonal antibody facility of the uo under the supervision of dr. The diagnosis of mantle cell lymphoma mcl can be difficult, especially when t11. Live image profiling of neural crest lineages in zebrafish. The transcription factor sox10 is a key regulator of. Pdf primary spinal opc culture system from adult zebrafish. These progenitor cells also give rise to the pigment, neuronal and glial cell lineages. To examine if the increased egfp reflects an upregulation of endogenous sox10 protein, we performed immunostaining with a sox10 antibody park et al. Chromatin immunoprecipitation chip assay of endogenous sox10 protein using anti sox10 antibody. Zebrafish danio rerio, a minnowlike freshwater fish, has emerged as a popular animal model for research and development. Meis3 is required for neural crest invasion of the gut during.
A zebrafish model for waardenburg syndrome type iv reveals. Sox10 mutations constitute less than 5% of hscr cases and are also associated with ws 20,40,44,45. The zebrafish drg phenotype shows many similarities to that in mouse, suggesting that an essential role for sox10 in drg development is conserved throughout vertebrates. The suppression of sox10 in melanoma cells activates tgf. Jun 14, 2011 the erbb3 gene is essential for the proper development of the neural crest nc and its derivative populations such as schwann cells. In particular, the sox10 protein is essential for the formation of nerves in the intestine enteric nerves and for the production of specialized cells called melanocytes. One factor proposed to regulate erbb3 is sox10, a member of the soxe family of transcription factors 20.
To this end, transverse sections through the telencephalon of tgolig2. It is also an important marker in malignant tumors such as melanoma, breast carcinoma, gliomas and benign tumors such as schwannomas. Sox17 antibody h is a high quality rabbit polyclonal recommended for detecting sox17 of mouse, rat and human origin by wb, ip, if and elisa. Sox10 directly modulates erbb3 transcription via an intronic. A guide for the laboratory use of zebrafish danio rerio, eugene, university of oregon press. Gfpba4 embryo at 16 hpf showing gfp expression in premigratory and early migrating neural crest cells. In december 2005, a cancer research team headed by keith cheng at.
Specificity of human sox10 antibody verified on a protein array containing target protein plus 383 other nonspecific proteins. Sox10 directly modulates erbb3 transcription via an intronic neural crest enhancer. Human monoclonal sox10 primary antibody for ihc p, elisa abin520385. About these antibodies all antibodies listed on this page were generated by researchers at the institute of neuroscience, university of oregon liu et al. We show that by external regulation of sox10 expression, we can rescue some aspects of the neural crest derived phenotypes in the charge model suggesting possible avenues of intervention in the future. Our results show significant 48 times enrichment over nonspecific antibody, indicating that the sox10 locus and, in particular, the sox10e2 regulatory element, was occupied by endogenous sox9, ets1, and cmyb proteins in the cranial region of hh8 to 10 chicken embryos fig. Histopathological study of the treatment of melasma lesions using a lowfluence qswitched 1064nm neodymium. Dorsal view of head and anterior trunk of a tg4725sox10. By 24 hpf, the otic vesicle of the sox10 colourless, cls mutant has no apparent morphological defects dutton et al. Full event breakdown with abstracts, speakers, registration and more. Normal rabbit igg was used as a negative ip control. Gfp antibody and by fluorescent in situ hybridisation with sox10 antisense probe, a marker of the oligodendrocyte lineage kuhlbrodt et al. The monoclonal antibodies were generated with balbc mice against adult zebrafish protein, and clones were selected based on the. Schwann cell precursors contribute to skeletal formation.
Neural crest progenitor cells are the main contributors to craniofacial cartilage and connective tissue of the vertebrate head. For detailed information, see the zebrafish model organism database. We offer sox10 antibodies for use in common research applications. Ordering fish lines, cdnas and the zebrafish book downloading zfin data in bulk linking directly to a zfin record citing zfin getting additional help antibody search tips mutant search tips gene expression search tips. Sox10 expression is highly conserved between all vertebrates characterised. The sox10 mo1 henceforth referred to as sox10 mo is predicted to block the translation and immunostaining of sox10 mo injected embryos with antisox10 antibody showed a dramatic reduction in the protein levels in 24hpf embryos supplementary material, fig. Detection of sox2 in ntera2 human cell line by flow cytometry.
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